neet-2018

NEET 2018

MEDICAL 2018 Previous Year

3 hDuration
632Total Marks
158Questions
3Sections
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  • Duration: 3 h. The timer starts as soon as you begin and cannot be paused.
  • Total questions: 158 across 3 section(s); maximum marks: 632.
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Paper Structure

Chemistry

Chemistry

Q1. mcq single +4 / 1
Which one of the following ions exhibits d-d transition and paramagnetism as well?
Q2. mcq single +4 / 1
Match the metal ions given in Column I with the spin magnetic moments of the ions given in Column II and assign the correct code : Column - I Column - II A. Co^(3+) (i) $$\sqrt 8 $$ B.M. B. Cr^(3+) (ii) $$\sqrt {35} $$ B.M. C. Fe^(3+) (iii) $$\sqrt 3 $$ B.M. D. Ni^(2+) (iv) $$\sqrt {24} $$ B.M. (v) $$\sqrt {15} $$ B.M.
Q3. mcq single +4 / 1
Consider the following species : CN^(+) , CN^(–) , NO and CN. Which one of these will have the highest bond order?
Q4. mcq single +4 / 1
In the structure of ClF~3~ , the number of lone pairs of electrons on central atom ‘Cl’ is
Q5. mcq single +4 / 1
The solubility of BaSO~4~ in water is 2.42 × 10^(–3) g L^(–1) at 298 K. The value of its solubility product (K~sp~) will be (Given molar mass of BaSO~4~ = 233 g mol^(–1))
Q6. mcq single +4 / 1
Following solutions were prepared by mixing different volumes of NaOH and HCl of different concentrations : A. 60 mL $${M \over {10}}$$ HCl + 40 mL $${M \over {10}}$$ NaOH B. 55 mL $${M \over {10}}$$ HCl + 45 mL $${M \over {10}}$$ NaOH C. 75 mL $${M \over {5}}$$ HCl + 25 mL $${M \over {5}}$$ NaOH D. 100 mL $${M \over {10}}$$ HCl + 100 mL $${M \over {10}}$$ NaOH pH of which one of them will be equal to 1?
Q7. mcq single +4 / 1
The correct difference between first and second order reactions is that
Q8. mcq single +4 / 1
When initial concentration of the reactant is doubled, the half-life period of a zero order reaction
Q9. mcq single +4 / 1
For the redox reaction, MnO~4~^(–) + C~2~O~4~ ^(2–) + H^(+) $$ \to $$ Mn^(2+) + CO~2~ + H~2~O The correct coefficients of the reactants for the balanced equation are
Q10. mcq single +4 / 1
The correct order of N-compounds in its decreasing order of oxidation states is
Q11. mcq single +4 / 1
The compound C~7~H~8~ undergoes the following reactions : The product ‘C’ is
Q12. mcq single +4 / 1
In which case is number of molecules of water maximum?
Q13. mcq single +4 / 1
A mixture of 2.3 g formic acid and 4.5 g oxalic acid is treated with conc. H~2~SO~4~ . The evolved gaseous mixture is passed through KOH pellets. Weight (in g) of the remaining product at STP will be
Q14. mcq single +4 / 1
Which one of the following conditions will favour maximum formation of the product in the reaction A~2(g)~ + B~2(g)~ ⇌ X~2(g)~ , $$\Delta $$~r~H = –X kJ ?
Q15. mcq single +4 / 1
Which of the following oxides is most acidic in nature?
Q16. mcq single +4 / 1
The correct order of atomic radii in group 13 elements is
Q17. mcq single +4 / 1
The bond dissociation energies of X~2~ , Y~2~ and XY are in the ratio of 1 : 0.5 : 1. $$\Delta $$H for the formation of XY is –200 kJ mol^(–1). The bond dissociation energy of X~2~ will be
Q18. mcq single +4 / 1
Which of the following statements is not true for halogens?
Q19. mcq single +4 / 1
Which one of the following elements is unable to form MF~6~^(3–) ion?
Q20. mcq single +4 / 1
The type of isomerism shown by the complex [CoCl~2~(en)~2~] is
Q21. mcq single +4 / 1
Iron carbonyl, Fe(CO)~5~ is
Q22. mcq single +4 / 1
The geometry and magnetic behaviour of the complex [Ni(CO)~4~] are
Q23. mcq multi +4 / 1
Which of the following is correct with respect to – I effect of the substituents? (R = alkyl)
Q24. mcq single +4 / 1
Which of the following carbocations is expected to be most stable?
Q25. mcq single +4 / 1
Which of the following molecules represents the order of hybridisation sp^(2) , sp^(2) , sp, sp from left to right atoms?
Q26. mcq single +4 / 1
Carboxylic acids have higher boiling points than aldehydes, ketones and even alcohols of comparable molecular mass. It is due to their
Q27. mcq single +4 / 1
Identify the major products P, Q and R in the following sequence of reactions :
Q28. mcq single +4 / 1
The compound A on treatment with Na gives B, and with PCl~5~ gives C. B and C react together to give diethyl ether. A, B and C are in the order
Q29. mcq single +4 / 1
Compound A, C~8~H~10~O, is found to react with NaOI (produced by reacting Y with NaOH) and yields a yellow precipitate with characteristic smell. A and Y are respectively
Q30. mcq single +4 / 1
In the reaction, the electrophile involved is
Q31. mcq single +4 / 1
Hydrocarbon (A) reacts with bromine by substitution to form an alkyl bromide which by Wurtz reaction is converted to gaseous hydrocarbon containing less than four carbon atoms. (A) is
Q32. mcq single +4 / 1
The difference between amylose and amylopectin is
Q33. mcq single +4 / 1
Which of the following compounds can form a zwitter ion?
Q34. mcq single +4 / 1
Consider the change in oxidation state of bromine corresponding to different emf values as shown in the given diagram : Then the species undergoing disproportionation is :
Q35. mcq single +4 / 1
Nitration of aniline in strong acidic medium also gives m-nitroaniline because
Q36. mcq single +4 / 1
Which one is a wrong statement?
Physics

Physics

Q1. mcq single +4 / 1
Unpolarised light is incident from air on a plane surface of a material of refractive index $$\mu $$. At a particular angle of incidence i, it is found that the reflected and refracted rays are perpendicular to each other. Which of the following options is correct for this situation?
Q2. mcq single +4 / 1
In Young’s double slit experiment the separation d between the slits is 2 mm, the wavelength $$\lambda $$ of the light used is 5896 $$\mathop A\limits^0 $$ and distance D between the screen and slits is 100 cm. It is found that the angular width of the fringes is 0.20^(o). To increase the fringe angular width to 0.21^(o) (with same $$\lambda $$ and D) the separation between the slits needs to be changed to
Q3. mcq single +4 / 1
Three objects, A : (a solid sphere), B : (a thin circular disk) and C : (a circular ring), each have the same mass M and radius R. They all spin with the same angular speed $$\omega $$ about their own symmetry axes. The amounts of work (W) required to bring them to rest, would satisfy the relation
Q4. mcq single +4 / 1
A solid sphere is in rolling motion. In rolling motion a body possesses translational kinetic energy (K~t~) as well as rotational kinetic energy (K~r~) simultaneously. The ratio K~t~ : (K~t~ + K~r~) for the sphere is
Q5. mcq single +4 / 1
A solid sphere is rotating freely about its symmetry axis in free space. The radius of the sphere is increased keeping its mass same. Which of the following physical quantities would remain constant for the sphere?
Q6. mcq single +4 / 1
The moment of the force, $$\overrightarrow F = 4\widehat i + 5\widehat j - 6\widehat k$$ at (2, 0, –3), about the point (2, –2, –2), is given by
Q7. mcq single +4 / 1
A student measured the diameter of a small steel ball using a screw gauge of least count 0.001 cm. The main scale reading is 5 mm and zero of circular scale division coincides with 25 divisions above the reference level. If screw gauge has a zero error of –0.004 cm, the correct diameter of the ball is
Q8. mcq single +4 / 1
The volume (V) of a monatomic gas varies with its temperature (T), as shown in the graph. The ratio of work done by the gas, to the heat absorbed by it, when it undergoes a change from state A to state B, is
Q9. mcq single +4 / 1
The efficiency of an ideal heat engine working between the freezing point and boiling point of water, is
Q10. mcq single +4 / 1
At what temperature will the rms speed of oxygen molecules become just sufficient for escaping from the Earth’s atmosphere? (Given : Mass of oxygen molecule (m) = 2.76 × 10^(–26) kg, Boltzmann’s constant k~B~ = 1.38 × 10^(–23) J K^(–1))
Q11. mcq single +4 / 1
A sample of 0.1 g of water at 100°C and normal pressure (1.013 × 10^(5) N m^(–2)) requires 54 cal of heat energy to convert to steam at 100°C. If the volume of the steam produced is 167.1 cc, the change in internal energy of the sample, is
Q12. mcq single +4 / 1
A small sphere of radius ‘r’ falls from rest in a viscous liquid. As a result, heat is produced due to viscous force. The rate of production of heat when the sphere attains its terminal velocity, is proportional to
Q13. mcq single +4 / 1
Two wires are made of the same material and have the same volume. The first wire has cross-sectional area A and the second wire has cross-sectional area 3A. If the length of the first wire is increased by $$\Delta $$l on applying a force F, how much force is needed to stretch the second wire by the same amount?
Q14. mcq single +4 / 1
The power radiated by a black body is P and it radiates maximum energy at wavelength, $$\lambda $$~0~ . If the temperature of the black body is now changed so that it radiates maximum energy at wavelength $${3 \over 4}{\lambda _0}$$, the power radiated by it becomes nP. The value of n is
Q15. mcq single +4 / 1
An electron falls from rest through a vertical distance h in a uniform and vertically upward directed electric field E. The direction of electric field is now reversed, keeping its magnitude the same. A proton is allowed to fall from rest in it through the same vertical distance h. The time of fall of the electron, in comparison to the time of fall of the proton is
Q16. mcq single +4 / 1
A toy car with charge q moves on a frictionless horizontal plane surface under the influence of a uniform electric field $$\overrightarrow E $$ . Due to the force q$$\overrightarrow E $$ , its velocity increases from 0 to 6 m s^(–1) in one second duration. At that instant the direction of the field is reversed. The car continues to move for two more seconds under the influence of this field. The average velocity and the average speed of the toy car between 0 to 3 seconds are respectively
Q17. mcq single +4 / 1
The kinetic energies of a planet in an elliptical orbit about the Sun, at positions A, B and C are K~A~, K~B~ and K~C~, respectively. AC is the major axis and SB is perpendicular to AC at the position of the Sun S as shown in the figure. Then
Q18. mcq single +4 / 1
If the mass of the Sun were ten times smaller and the universal gravitational constant were ten times larger in magnitude, which of the following is not correct?
Q19. mcq single +4 / 1
The electrostatic force between the metal plates of an isolated parallel plate capacitor C having a charge Q and area A, is
Q20. mcq single +4 / 1
The refractive index of the material of a prism is $$\sqrt 2 $$ and the angle of the prism is 30°. One of the two refracting surfaces of the prism is made a mirror inwards, by silver coating. A beam of monochromatic light entering the prism from the other face will retrace its path (after reflection from the silvered surface) if its angle of incidence on the prism is
Q21. mcq single +4 / 1
An astronomical refracting telescope will have large angular magnification and high angular resolution, when it has an objective lens of
Q22. mcq single +4 / 1
An object is placed at a distance of 40 cm from a concave mirror of focal length 15 cm. If the object is displaced through a distance of 20 cm towards the mirror, the displacement of the image will be
Q23. mcq single +4 / 1
A pendulum is hung from the roof of a sufficiently high building and is moving freely to and fro like a simple harmonic oscillator. The acceleration of the bob of the pendulum is 20 m s^(–2) at a distance of 5 m from the mean position. The time period of oscillation is
Q24. mcq single +4 / 1
In the combination of the following gates the output Y can be written in terms of inputs A and B as
Q25. mcq single +4 / 1
In a p-n junction diode, change in temperature due to heating
Q26. mcq single +4 / 1
Current sensitivity of a moving coil galvanometer is 5 div/mA and its voltage sensitivity (angular deflection per unit voltage applied) is 20 div/V. The resistance of the galvanometer is
Q27. mcq single +4 / 1
A metallic rod of mass per unit length 0.5 kg m^(–1) is lying horizontally on a smooth inclined plane which makes an angle of 30° with the horizontal. The rod is not allowed to slide down by flowing a current through it when a magnetic field of induction 0.25 T is acting on it in the vertical direction. The current flowing in the rod to keep it stationary is
Q28. mcq single +4 / 1
When the light of frequency 2$${\upsilon _0}$$ (where $${\upsilon _0}$$ is threshold frequency), is incident on a metal plate, the maximum velocity of electrons emitted is v~1~ . When the frequency of the incident radiation is increased to 5$${\upsilon _0}$$ , the maximum velocity of electrons emitted from the same plate is v~2~ . The ratio of v~1~ to v~2~ is
Q29. mcq single +4 / 1
An electron of mass m with an initial velocity $$\overrightarrow v = {v_0}\widehat i$$ (v~0~ > 0) enters an electric field $$\overrightarrow E = - {\overrightarrow E _0}\widehat i$$ (E~0~ = constant > 0) at t = 0. If $$\lambda $$~0~ is its de-Broglie wavelength initially, then its de- Broglie wavelength at time t is
Q30. mcq single +4 / 1
The ratio of kinetic energy to the total energy of an electron in a Bohr orbit of the hydrogen atom, is
Q31. mcq single +4 / 1
A moving block having mass m, collides with another stationary block having mass 4m. The lighter block comes to rest after collision. When the initial velocity of the lighter block is v, then the value of coefficient of restitution (e) will be
Q32. mcq single +4 / 1
The fundamental frequency in an open organ pipe is equal to the third harmonic of a closed organ pipe. If the length of the closed organ pipe is 20 cm, the length of the open organ pipe is
Q33. mcq single +4 / 1
A tuning fork is used to produce resonance in a glass tube. The length of the air column in this tube can be adjusted by a variable piston. At room temperature of 27°C two successive resonances are produced at 20 cm and 73 cm of column length. If the frequency of the tuning fork is 320 Hz, the velocity of sound in air at 27°C is
Q34. mcq single +4 / 1
Which one of the following statements is incorrect?
Q35. mcq single +4 / 1
A block of mass m is placed on a smooth inclined wedge ABC of inclination q as shown in the figure. The wedge is given an acceleration ‘a’ towards the right. The relation between a and q for the block to remain stationary on the wedge is
Q36. mcq single +4 / 1
A thin diamagnetic rod is placed vertically between the poles of an electromagnet. When the current in the electromagnet is switched on, then the diamagnetic rod is pushed up, out of the horizontal magnetic field. Hence the rod gains gravitational potential energy. The work required to do this comes from
Q37. mcq single +4 / 1
A body initially at rest and sliding along a frictionless track from a height h (as shown in the figure) just completes a vertical circle of diameter AB = D. The height h is equal to
Q38. mcq single +4 / 1
A set of n equal resistors, of value R each, are connected in series to a battery of emf E and internal resistance R. The current drawn is I. Now, the n resistors are connected in parallel to the same battery. Then the current drawn from battery becomes 10I. The value of n is
Q39. mcq single +4 / 1
A battery consists of a variable number n of identical cells (having internal resistance r each) which are connected in series. The terminals of the battery are short-circuited and the current I is measured. Which of the graphs shows the correct relationship between I and n?
Q40. mcq single +4 / 1
A carbon resistor of (47 $$ \pm $$ 4.7) k$$\Omega $$ is to be marked with rings of different colours for its identification. The colour code sequence will be
Q41. mcq single +4 / 1
An EM wave is propagating in a medium with a velocity $$\overrightarrow v = v\widehat i$$. The instantaneous oscillating electric field of this em wave is along +y axis. Then the direction of oscillating magnetic field of the em wave will be along
Q42. mcq single +4 / 1
An inductor 20 mH, a capacitor 100 $$\mu $$F and a resistor 50 $$\Omega $$ are connected in series across a source of emf, V = 10 sin 314 t. The power loss in the circuit is
Q43. mcq single +4 / 1
The magnetic potential energy stored in a certain inductor is 25 mJ, when the current in the inductor is 60 mA. This inductor is of inductance
Biology

Biology

Q1. mcq single +4 / 1
Conversion of milk to curd improves its nutritional value by increasing the amount of
Q2. mcq single +4 / 1
What type of ecological pyramid would be obtained with the following data? Secondary consumer : 120 g Primary consumer : 60 g Primary producer : 10 g
Q3. mcq single +4 / 1
A woman has an X-linked condition on one of her X chromosomes. This chromosome can be inherited by
Q4. mcq single +4 / 1
Which of the following characteristics represent 'Inheritance of blood groups' in humans? a. Dominance b. Co-dominance c. Multiple allele d. Incomplete dominance e. Polygenic inheritance
Q5. mcq single +4 / 1
Which of the following pairs is wrongly matched?
Q6. mcq single +4 / 1
Which of the following features is used to identify a male cockroach from a female cockroach?
Q7. mcq single +4 / 1
Which of the following is not a product of light reaction of photosynthesis?
Q8. mcq single +4 / 1
Identify the vertebrate group of animals characterised by crop and gizzard in its digestive system.
Q9. mcq single +4 / 1
Which one of these animals is not a homeotherm?
Q10. mcq single +4 / 1
Which of the following animals does not undergo metamorphosis?
Q11. mcq single +4 / 1
Which of the following statements is correct?
Q12. mcq single +4 / 1
Which one is wrongly matched?
Q13. mcq single +4 / 1
Which of the following is an occupational respiratory disorder?
Q14. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I Column II A. Tidal volume (i) 2500 – 3000 mL B. Inspiratory reserve volume (ii) 1100 – 1200 mL C. Expiratory reserve volume (iii) 500 – 550 mL D. Residual volume (iv) 1000 – 1100 mL
Q15. mcq single +4 / 1
Which of the following options correctly represents the lung conditions in asthma and emphysema, respectively?
Q16. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I Column II A. Herbarium (i) It is a place having a collection of preserved plants and animals. B. Key (ii) A list that enumerates methodically all the species found in an area with brief description aiding identification. C. Museum (iii) Is a place where dried and pressed plant specimens mounted on sheets are kept. D. Catalogue (iv) A booklet containing a list of characters and their alternates which are helpful in identification of various taxa.
Q17. mcq single +4 / 1
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
Q18. mcq single +4 / 1
In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is
Q19. mcq single +4 / 1
Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
Q20. mcq single +4 / 1
A 'new' variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to
Q21. mcq single +4 / 1
Nissl’s bodies are mainly composed of
Q22. mcq single +4 / 1
Which of the following structures or regions is incorrectly paired with its functions?
Q23. mcq single +4 / 1
The transparent lens in the human eye is held in its place by
Q24. mcq single +4 / 1
Plants having little or no secondary growth are
Q25. mcq single +4 / 1
Stomata in grass leaf are
Q26. mcq single +4 / 1
Secondary xylem and phloem in dicot stem are produced by
Q27. mcq single +4 / 1
Casparian strips occur in
Q28. mcq single +4 / 1
Which of the following is true for nucleolus?
Q29. mcq single +4 / 1
The Golgi complex participates in
Q30. mcq single +4 / 1
Select the incorrect match.
Q31. mcq single +4 / 1
Which of the following events does not occur in rough endoplasmic reticulum?
Q32. mcq single +4 / 1
Select the correct match :
Q33. mcq single +4 / 1
AGGTATCGCAT is a sequence from the coding strand of a gene. What will be the corresponding sequence of the transcribed mRNA?
Q34. mcq single +4 / 1
Many ribosomes may associate with a single mRNA to form multiple copies of a polypeptide simultaneously. Such strings of ribosomes are termed as
Q35. mcq single +4 / 1
All of the following are part of an operon except
Q36. mcq single +4 / 1
Select the correct match :
Q37. mcq single +4 / 1
Select the correct statement :
Q38. mcq single +4 / 1
The experimental proof for semi-conservative replication of DNA was first shown in a
Q39. mcq single +4 / 1
Which part of poppy plant is used to obtain the drug “smack”?
Q40. mcq single +4 / 1
In which disease does mosquito transmitted pathogen cause chronic inflammation of lymphatic vessels?
Q41. mcq single +4 / 1
Which of the following is not an autoimmune disease?
Q42. mcq single +4 / 1
Natality refers to
Q43. mcq single +4 / 1
Pneumatophores occur in
Q44. mcq single +4 / 1
Which one of the following plants shows a very close relationship with a species of moth, where none of the two can complete its life cycle without the other?
Q45. mcq single +4 / 1
Which one of the following population interactions is widely used in medical science for the production of antibiotics?
Q46. mcq single +4 / 1
Niche is
Q47. mcq single +4 / 1
In a growing population of a country,
Q48. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I Column II A. Tricuspid valve (i) Between left atrium and left ventricle B. Bicuspid valve (ii) Between right ventricle and pulmonary artery C. Semilunar valve (iii) Between right atrium and right ventricle
Q49. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I Column II A. Fibrinogen (i) Osmotic balance B. Globulin (ii) Blood clotting C. Albumin (iii) Defence mechanism
Q50. mcq single +4 / 1
The stage during which separation of the paired homologous chromosomes begins is
Q51. mcq single +4 / 1
The difference between spermiogenesis and spermiation is
Q52. mcq single +4 / 1
Hormones secreted by the placenta to maintain pregnancy are
Q53. mcq single +4 / 1
The amnion of mammalian embryo is derived from
Q54. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column - I Column - II A. Proliferative phase (i) Breakdown of endometrial lining B. Secretory Phase (ii) Follicular Phase C. Menstruation (iii) Luteal Phase
Q55. mcq single +4 / 1
Which of the following hormones can play a significant role in osteoporosis?
Q56. mcq single +4 / 1
Which of the following is an amino acid derived hormone?
Q57. mcq single +4 / 1
Pollen grains can be stored for several years in liquid nitrogen having a temperature of
Q58. mcq single +4 / 1
All of the following are included in ‘ex-situ conservation’ except
Q59. mcq single +4 / 1
Which of these statements is incorrect?
Q60. mcq single +4 / 1
What is the role of NAD^(+) in cellular respiration?
Q61. mcq single +4 / 1
Calcium is important in skeletal muscle contraction because it
Q62. mcq single +4 / 1
After karyogamy followed by meiosis, spores are produced exogenously in
Q63. mcq single +4 / 1
Select the wrong statement.
Q64. mcq single +4 / 1
Which among the following is not a prokaryote?
Q65. mcq single +4 / 1
Ciliates differ from all other protozoans in
Q66. mcq single +4 / 1
Which of the following organisms are known as chief producers in the oceans?
Q67. mcq single +4 / 1
Oxygen is not produced during photosynthesis by
Q68. mcq single +4 / 1
Among the following sets of examples for divergent evolution, select the incorrect option.
Q69. mcq single +4 / 1
According to Hugo de Vries, the mechanism of evolution is
Q70. mcq single +4 / 1
The similarity of bone structure in the forelimbs of many vertebrates is an example of
Q71. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I Column II A. Glycosuria (i) Accumulation of uric acid in joints B. Gout (ii) Mass of crystallised salts within the kidney C. Renal calculi (iii) Inflammation in glomeruli D. Glomerular nephritis (iv) Presence of glucose in urine
Q72. mcq single +4 / 1
Match the items given in column I with those in column II and select the correct option given below. Column I (Function) Column II (Part of excretory system) A. Ultrafiltration (i) Henle’s loop B. Concentration of urine (ii) Ureter C. Transport of urine (iii) Urinary bladder D. Storage of urine (iv) Malpighian corpuscle (v) Proximal convoluted tubule
Q73. mcq single +4 / 1
The contraceptive ‘Saheli’
Q74. mcq single +4 / 1
Winged pollen grains are present in
Q75. mcq single +4 / 1
Double fertilization is
Q76. mcq single +4 / 1
Which of the following has proved helpful in preserving pollen as fossils?
Q77. mcq single +4 / 1
Sweet potato is a modified
Q78. mcq single +4 / 1
The two functional groups characteristic of sugars are
Q79. mcq single +4 / 1
The correct order of steps in Polymerase Chain Reaction (PCR) is